首都大学東京 理工学研究科分子物質化学専攻　生物化学研究室

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原著論文

2015

  • Yamauchi Y, Nobe Y, Izumikawa K, Higo D, Yamagishi Y, Takahashi N, Nakayama　H, Isobe T, Taoka M.
    A mass spectrometry-based method for direct determination of pseudouridine in RNA
    Nucleic Acids Res. 2015 Nov 27. DOI: 10.1093/nar/gkv1462
  • Taoka M, Nobe Y, Hori M, Takeuchi A, Masaki S, Yamauchi Y, Nakayama H, Takahashi N, Isobe T.
    A mass spectrometry-based method for comprehensive quantitative determination of post-transcriptional RNA modifications: the complete chemical structure of Schizosaccharomyces pombe ribosomal RNAs
    Nucleic Acids Res. 2015 May 26. pii: gkv560. [Epub ahead of print]
  • Iguchi T, Aoki K, Ikawa T, Taoka M, Taya C, Yoshitani H, Toma-Hirano M, Koiwai O, Isobe T, Kawamoto H, Masai H, Miyatake S.
    BTB-ZF Protein Znf131 Regulates Cell Growth of Developing and Mature T Cells.
    J Immunol. 2015 Jul 1. pii: 1500602. [Epub ahead of print]
  • Yoshikawa H, Ishikawa H, Izumikawa K, Miura Y, Hayano T, Isobe T, Simpson RJ, Takahashi N.
    Human nucleolar protein Nop52 (RRP1/NNP-1) is involved in site 2 cleavage in internal transcribed spacer 1 of pre-rRNAs at early stages of ribosome biogenesis.
    Nucleic Acids Res. 2015 May 12. pii: gkv470. [Epub ahead of print]
  • Nakayama H, Yamauchi Y, Taoka M, Isobe T.
    Direct identification of human cellular microRNAs by nanoflow liquid chromatography-high-resolution tandem mass spectrometry and database searching.
    Anal Chem. 2015 Mar 3;87(5):2884-91. doi: 10.1021/ac504378s..
  • Tanji H, Ohto U, Shibata T, Taoka M, Yamauchi Y, Isobe T, Miyake K, Shimizu T.
    Toll-like receptor 8 senses degradation products of single-stranded RNA.
    Nat Struct Mol Biol. 2015 Feb;22(2):109-15. doi: 10.1038/nsmb.2943..
  • Kurata T, Nakanishi S, Hashimoto M, Taoka M, Yamazaki Y, Isobe T, Kato J.
    Novel essential gene Involved in 16S rRNA processing in Escherichia coli.
    J Mol Biol. 2015 Feb 27;427(4):955-65. doi: 10.1016/j.jmb.2014.12.013.

2014

  • Asano T, Taoka M, Yamauchi Y, Craig Everroad R, Seto Y, Isobe T, Kamo M, Chosa N.
    Re-examination of a α-chymotrypsin-solubilized laccase in the pupal cuticle of the silkworm, Bombyx mori: Insights into the regulation system for laccase activation during the ecdysis process.
    Insect Biochem Mol Biol. 2014 Oct 30;55C:61-69. doi: 10.1016/j.ibmb.2014.10.004.
  • Taoka M, Ishikawa D, Nobe Y, Ishikawa H, Yamauchi Y, Terukina G, Nakayama H, Hirota K, Takahashi N, Isobe T.
    RNA Cytidine Acetyltransferase of Small-Subunit Ribosomal RNA: Identification of Acetylation Sites and the Responsible Acetyltransferase in Fission Yeast, Schizosaccharomyces pombe,
    PLoS One, 2014 9 (11): e112156. doi: 10.1371/journal.pone.0112156.
  • Saito Y, Takeda J, Adachi K, Nobe Y, Kobayashi J, Hirota K, Oliveira D. V, Taoka M, Isobe, T.
    RNase MRP Cleaves pre-tRNASer-Met in the tRNA Maturation Pathway,
    PLoS One, 2014; 9 (11): e112488. doi: 10.1371/journal.pone.0112488.
  • Yamazaki D, Horiuchi J, Ueno K, Ueno T, Saeki S, Matsuno M, Nagano S, Miyashita T, Hirano Y, Nishikawa H, Taoka M, Yamauchi Y, Isobe T, Honda Y, Kodama T, Masuda T, Saitoe M,
    Glial dysfunction causes age-related memory impairment in Drosophila,
    Neuron, 2014; 84 (4): 753-63. doi: 10.1016/j.neuron.2014.09.039.
  • Manabe Y, Takagi M, Nakamura-Yamada M, Goto-Inoue N, Taoka M, Isobe T, Fujii NL,
    Redox proteins are constitutively secreted by skeletal muscle. J Physiol Sci. 2014 Sep 10. [Epub ahead of print]
  • Maesaki R, Satoh R, Taoka M, Kanaba T, Asano T, Fujita C, Fujiwara T, Ito Y, Isobe T, Hakoshima T, Maenaka K, Mishima M.
    Efficient and cost effective production of active-form human PKB using silkworm larvae.
    Sci Rep. 2014 Aug 15;4:6016. doi: 10.1038/srep06016.
  • Abdelhamid RF, Plessy C, Yamauchi Y, Taoka M, de Hoon M, Gingeras TR, Isobe T, Carninci P.
    Multiplicity of 5' cap structures present on short RNAs. PLoS One. 2014 Jul 31;9(7):e102895. doi: 10.1371/journal.pone.0102895.
  • Taoka M, Morofuji N, Yamauchi Y, Ojima H, Kubota D, Terukina G, Nobe Y, Nakayama H, Takahashi N, Kosuge T, Isobe T, Kondo T.
    Global PROTOMAP Profiling to Search for Biomarkers of Early-Recurrent Hepatocellular Carcinoma.
    J Proteome Res. 2014 Jul 7. [Epub ahead of print]
  • Unno J, Itaya A, Taoka M, Sato K, Tomida J, Sakai W, Sugasawa K, Ishiai M, Ikura T, Isobe T, Kurumizaka H, Takata M.
    FANCD2 binds CtIP and regulates DNA-end resection during DNA interstrand crosslink repair.
    Cell Rep. 2014 May 22;7(4):1039-47. doi: 10.1016/j.celrep.2014.04.005.
  • Tanabe K, Yamazaki H, Inaguma Y, Asada A, Kimura T, Takahashi J, Taoka M, Ohshima T, Furuichi T, Isobe T, Nagata K, Shirao T, Hisanaga S.
    Phosphorylation of drebrin by cyclin-dependent kinase 5 and its role in neuronal migration.
    PLoS One. 2014 Mar 17;9(3):e92291. doi: 10.1371/journal.pone.0092291.
  • Miyazawa N, Yoshikawa H, Magae S, Ishikawa H, Izumikawa K, Terukina G, Suzuki A, Nakamura-Fujiyama S, Miura Y, Hayano T, Komatsu W, Isobe T, Takahashi N.
    Human cell growth regulator Ly-1 antibody reactive homologue accelerates processing of preribosomal RNA.
    Genes Cells. 2014 Apr;19(4):273-86. doi: 10.1111/gtc.12129.
  • Miyatake S, Manabe Y, Inagaki A, Furuichi Y, Takagi M, Taoka M, Isobe T, Hirota K, Fujii NL.
    Macrophage migration inhibitory factor diminishes muscle glucose transport induced by insulin and AICAR in a muscle type-dependent manner.
    Biochem Biophys Res Commun. 2014 Feb 21;444(4):496-501. doi: 10.1016/j.bbrc.2014.01.089
  • Ishikawa H, Nobe Y, Izumikawa K, Yoshikawa H, Miyazawa N, Terukina G, Kurokawa N, Taoka M, Yamauchi Y, Nakayama H, Isobe T, Takahashi N.
    Identification of truncated forms of U1 snRNA reveals a novel RNA degradation pathway during snRNP biogenesis.
    Nucleic Acids Res. 2014 Feb;42(4):2708-24. doi: 10.1093/nar/gkt1271.

2013

  • Kaji H, Isobe T.
    Stable isotope labeling of N-glycosylated peptides by enzymatic deglycosylation for mass spectrometry-based glycoproteomics.
    Methods Mol Biol. 951: 217-27 (2013). doi: 10.1007/978-1-62703-146-2_14.
  • Kimura T, Tsutsumi K, Taoka M, Saito T, Masuda-Suzukake M, Ishiguro K, Plattner F, Uchida T, Isobe T, Hasegawa M, Hisanaga S.　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　
    Isomerase Pin1 stimulates dephosphorylation of tau protein at cyclin-dependent kinase (Cdk5)-dependent Alzheimer phosphorylation sites.　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　
    J Biol Chem. 288 (11): 7968-7977 (2013). doi: 10.1074/jbc.M112.433326.
  • Asano T, Taoka M, Shinkawa T, Yamauchi Y, Isobe T, Sato D.
    Identification of a cuticle protein with unique repeated motifs in the silkworm, Bombyx mori.
    Insect Biochem Mol Biol. 3 (4): 344-351 (2013). doi: 10.1016/j.ibmb.2013.01.001.
  • Ichimura T, Taoka M, Shoji I, Kato H, Sato T, Hatakeyama S, Isobe T, Hachiya N.
    14-3-3 proteins sequester a pool of soluble TRIM32 ubiquitin ligase to repress autoubiquitylation and cytoplasmic body formation.　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　
    J Cell Sci. 126 (Pt 9): 2014-2026 (2013). doi: 10.1242/jcs.122069.
  • Saito Y, Takeda J, Okada M, Kobayashi J, Kato A, Hirota K, Taoka M, Matsumoto T, Komatsu K, Isobe T.
    The proteasome factor Bag101 binds to Rad22 and suppresses homologous recombination.　　　　
    Sci Rep. 3: 2022 (2013). doi: 10.1038/srep02022
  • Abiko M, Furuta K, Yamauchi Y, Fujita C, Taoka M, Isobe T, Okamoto T.
    Identification of proteins enriched in rice egg or sperm cells by single-cell proteomics.　　　　　　　　　　　　　　　　　PLoS One. 8(7): e69578 (2013). doi: 10.1371/journal.pone.0069578.
  • Yamauchi Y, Taoka M, Nobe Y, Izumikawa K, Takahashi N, Nakayama H, Isobe T.
    Denaturing reversed phase liquid chromatographic separation of non-coding ribonucleic acids on macro-porous polystyrene-divinylbenzene resins.
    J Chromatogr A. 1312: 87-92 (2013). doi: 10.1016/j.chroma.2013.09.021.

2012

  • Uematsu T, Konishi C, Hoshino D, Han X, Tomari T, Egawa N, Takada Y, Isobe T, Seiki M, Koshikawa N.
    Identification of proteins that associate with integrin α2 by proteomic analysis in human fibrosarcoma HT-1080 cells.
    J Cell Physiol. 7 (8): 3072-3079 (2012). doi: 10.1002/jcp.23054.
  • Okada M, Hozumi Y, Ichimura T, Tanaka T, Hasegawa H, Yamamoto M, Takahashi N, Iseki K, Yagisawa H, Shinkawa T, Isobe T, Goto K.
    Interaction of nucleosome assembly proteins abolishes nuclear localization of DGKζ by attenuating its association with importins.　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　
    Exp Cell Res. 317 (20): 2853-2863 (2011). doi: 10.1016/j.yexcr.2011.09.014.
  • Harada E, Nakagawa J, Asano T, Taoka M, Sorimachi H, Ito Y, Aigaki T, Matsuo T.
    Functional evolution of duplicated odorant-binding protein genes, Obp57d and Obp57e, in Drosophila.
    PLoS One. 7 (1): e29710 (2012). doi: 10.1371/journal.pone.0029710.
  • Kaji H, Shikanai T, Sasaki-Sawa A, Wen H, Fujita M, Suzuki Y, Sugahara D, Sawaki H, Yamauchi Y, Shinkawa T, Taoka M, Takahashi N, Isobe T, Narimatsu H.
    Large-scale identification of N-glycosylated proteins of mouse tissues and construction of a glycoprotein database, GlycoProtDB.　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　
    J Proteome Res. 11 (9): 4553-4566 (2012). doi: 10.1021/pr300346c.

2011

  • Takahashi K, Suzuki T, Nishii W, Kubota K, Shibata C, Isobe T, Dohmae N. 　　　　　　　　　　　　　　
    A cysteine endopeptidase ("dionain") is involved in the digestive fluid of Dionaea muscipula (Venus's fly-trap).
    Biosci Biotechnol Biochem. 75 (2): 346-348 (2011).
  • Nakayama H, Takahashi N, Isobe T. 　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　
    Informatics for mass spectrometry-based RNA analysis.　　　　　　　　　　　　　　　　　　　　　　　　
    Mass Spectrom Rev. 30 (6): 1000-1012 (2011). doi: 10.1002/mas.20325. Review.
  • Nakatsu Y, Sakoda H, Kushiyama A, Zhang J, Ono H, Fujishiro M, Kikuchi T, Fukushima T, Yoneda M, Ohno H, Horike N, Kanna M, Tsuchiya Y, Kamata H, Nishimura F, Isobe T, Ogihara T, Katagiri H, Oka Y, Takahashi S, Kurihara H, Uchida T, Asano T.
    Peptidyl-prolyl cis/trans isomerase NIMA-interacting 1 associates with insulin receptor substrate-1 and enhances insulin actions and adipogenesis.　　　　　　　　　　　　　　　　　　　　　　　　　　
    J Biol Chem. 286 (23): 20812-20822 (2011). doi: 10.1074/jbc.M110.206904.
  • Yoshikawa H, Komatsu W, Hayano T, Miura Y, Homma K, Izumikawa K, Ishikawa H, Miyazawa N, Tachikawa H, Yamauchi Y, Isobe T, Takahashi N. 　　　　　　　　　　　　　　　　　　　　　　　　　　　
    Splicing factor 2-associated protein p32 participates in ribosome biogenesis by regulating the binding of Nop52 and fibrillarin to preribosome particles.
    Mol Cell Proteomics (8): M110.006148 (2011). doi: 10.1074/mcp.M110.006148.

2010

  • Asaoka Y, Kanai F, Ichimura T, Tateishi K, Tanaka Y, Ohta M, Seto M, Tada M, Ijichi H, Ikenoue T, Kawabe T, Isobe T, Yaffe MB, Omata M
    Identification of a suppressive mechanism for Hedgehog signaling through a novel interaction of Gli with 14-3-3
    J Biol Chem. 285 (6): 4185-4194 (2010). doi: 10.1074/jbc.M109.038232.
  • Tando T, Ishizaka A, Watanabe H, Ito T, Iida S, Haraguchi T, Mizutani T, Izumi T, Isobe T, Akiyama T, Inoue J, Iba H.　
    Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway. 　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　
    J Biol Chem. 285 (29): 21951-21960. (2010) doi: 10.1074/jbc.M109.087783.
  • Nakatsu Y, Sakoda H, Kushiyama A, Ono H, Fujishiro M, Horike N, Yoneda M, Ohno H, Tsuchiya Y, Kamata H, Tahara H, Isobe T, Nishimura F, Katagiri H, Oka Y, Fukushima T, Takahashi S, Kurihara H, Uchida T, Asano T.
    Pin1 associates with and induces translocation of CRTC2 to the cytosol, thereby suppressing cAMP-responsive element transcriptional activity.
    J Biol Chem. 285 (43): 33018-33027 (2010). doi: 10.1074/jbc.M110.137836.
  • Taoka M, Ikumi M, Nakayama H, Masaki S, Matsuda R, Nobe Y, Yamauchi Y, Takeda J, Takahashi N, Isobe T.
    In-gel digestion for mass spectrometric characterization of RNA from fluorescently stained polyacrylamide gels.
    Anal Chem. 82 (18): 7795-7803 (2010). doi: 10.1021/ac101623j.

2009

  • Nozumi M, Togano T, Takahashi-Niki K, Lu J, Honda A, Taoka M, Shinkawa T, Koga H, Takeuchi K, Isobe T, Igarashi M..
    Identification of functional marker proteins in the mammalian growth cone.
    Proc Natl Acad Sci U S A. 106(40):17211-6. (2009)
  • Taoka M, Yamauchi Y, Nobe Y, Masaki S, Nakayama H, Ishikawa H, Takahashi N, Isobe T..
    An analytical platform for mass spectrometry-based identification and chemical analysis of RNA in ribonucleoprotein complexes..
    Nucleic Acids Res. .(2009）
  • Niiya D, Egawa N, Sakamoto T, Kikkawa Y, Shinkawa T, Isobe T, Koshikawa N, Seiki M.
    Identification and characterization of Lutheran blood group glycoprotein as a new substrate of membrane-type 1 matrix
    J Biol Chem. 284(40):27360-9.(2009)
  • Okada M, Okawa K, Isobe T, Fukagawa T..
    CENP-H-containing complex facilitates centromere deposition of CENP-A in cooperation with FACT and CHD1.
    Mol Biol Cell. 20(18):3986-95.(2009)
  • Fujiyama-Nakamura S, Ito S, Sawatsubashi S, Yamauchi Y, Suzuki E, Tanabe M, Kimura S, Murata T, Isobe T, Takeyama K, Kato S.
    BTB protein, dKLHL18/CG3571, serves as an adaptor subunit for a dCul3 ubiquitin ligase complex..
    Genes Cells. 14(8):965-73. (2009)
  • Tomari T, Koshikawa N, Uematsu T, Shinkawa T, Hoshino D, Egawa N, Isobe T, Seiki M..
    High throughput analysis of proteins associating with a proinvasive MT1-MMP in human malignant melanoma A375 cells..
    Cancer Sci. 100(7):1284-90. (2009)
  • Aoki T, Ichimura S, Itoh A, Kuramoto M, Shinkawa T, Isobe T, Tagaya M.
    Identification of the neuroblastoma-amplified gene product as a component of the syntaxin 18 complex implicated in Golgi-to-endoplasmic reticulum retrograde transport.
    Mol Biol Cell. 20(11):2639-49. (2009)
  • Fujiyama-Nakamura S, Yoshikawa H, Homma K, Hayano T, Tsujimura-Takahashi T, Izumikawa K, Ishikawa H, Miyazawa N, Yanagida M, Miura Y, Shinkawa T, Yamauchi Y, Isobe T, Takahashi N
    Parvulin (Par14), a peptidyl-prolyl cis-trans isomerase, is a novel rRNA processing factor that evolved in the metazoan lineage.
    Mol Cell Proteomics. 8(7):1552-65. (2009)
  • Matsunaga K, Saitoh T, Tabata K, Omori H, Satoh T, Kurotori N, Maejima I, Shirahama-Noda K, Ichimura T, Isobe T, Akira S, Noda T, Yoshimori T
    Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages.
    Nat Cell Biol11(4):385-96. (2009)
  • Nakayama H, Akiyama M, Taoka M, Yamauchi Y, Nobe Y, Ishikawa H, Takahashi N, Isobe T.
    Ariadne: a database search engine for identification and chemical analysis of RNA using tandem mass spectrometry data
    Nucleic Acids Res. 37(6):e47. (2009)
PAGETOP

2008

  • Hayano T, Yamauchi Y, Asano K, Tsujimura T, Hashimoto S, Isobe T and Takahashi N.
    Automated SPR-LC-MS/MS system for protein interaction analysis.
    J. Proteome Res. 7(9), 4183-90 (2008）
  • Izumikawa K, Yanagida M, Hayano T, Tachikawa H, Komatsu W, Shimamoto A, Futami K, Furuichi Y, Shinkawa T, Yamauchi Y, Isobe T and Takahashi N.
    Association of human DNA helicase RecQ5beta with RNA polymerase II and its possible role in trascription.
    Biochem. J. 413(3), 505-16 (2008)
  • Ito T, Watanabe H, Yamamichi N, Kondo S, Tando T, Haraguchi T, Mizutani T, Sakurai K, Fujita S, Izumi T, Isobe T and Iba H.
    Brm transactivates the telomerase reverse transcriptase (TERT) gene and modulates the splicing patterns of its transcripts in concert with p54 nrb.
    Biochem J. 411(1), 201-9 (2008)
PAGETOP

2007

  • Taniguchi M, Taoka M, Itakura M, Asada A, Saito T, Kinoshita M, Takahashi M, Isobe T and Hisanaga S.
    Phosphorylation of adult type Sep5 (CDCrel-1) by cyclin-dependent kinase 5 inhibits interaction with syntaxin-1.
    J. Biol. Chem. 282(11),7869-7876 (2007）
  • Uchiumi T, Shinkawa T, Isobe T and Okamoto T.
    Identification of the major protein components of rice egg cells.
    J. Plant Res. 120(4), 575-579 (2007)
  • Kakiuchi K, Yamauchi Y, Taoka M, Iwago M, Fujita T, Ito T, Song SY, Sakai A, Isobe T, and Ichimura T.
    Proteomic analysis of in vivo 14-3-3 interactions in the yeast Saccharomyces cerevisiae.
    Biochemistry 46, 7781-92 (2007)
  • Tanaka Y, Imamura J, Kanai F, Ichimura T, Isobe T, Koike M, Kudo Y, Tateishi K, Ikenoue T, Ijichi H, Yamaji Y, Yoshida H, Kawabe T, and Omata M.
    Runx3 interacts with DNA repair protein Ku70.
    Exp Cell Res. 313(15), 3251-60 (2007)
  • Kaji H, Kamiie J, Kawakami H, Kido K, Yamauchi Y, Shinkawa T, Taoka M, Takahashi N and Isobe T.
    Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins.
    Mol Cell Proteomics 6 (12), 2100-2109 (2007)
  • Sato Y, Taoka M, Sugiyama N, Kubo K, Fuchigami T, Asada A, Saito T, Nakajima K, Isobe T and Hisanaga S.
    Regulation of the interaction of Disabled-1 with CIN85 by phosphorylation with Cyclin-dependent kinase 5.
    Genes Cells. 12 (12), 1315-1327 (2007)
  • Tanaka Y, Osaka T, Hattori A, Murakani Y, Matsufuji T, Matsufuji S, Shinkawa T, Isobe T and Mizutani T.
    SLA/LP, an Antigen in Autoimmune Hepatitis Patients: Influence on Selenocysteine Synthesis and its Complex with HSP70.
    J. Health Sci. 53, 682-691 (2007)
PAGETOP

2006

  • Fukunishi Y, Hojo S, and Nakamura H.
    An efficient in silico screening method based on the protein-compound affinity matrix and its application to the design of a focused library for cytochrome P450 (CYP) ligands.
    J Chem Inf Model. 46(6), 2610-22 (2006)
  • Nagano K, Itagaki C, Izumi T., Nunomura N, Soda Y, Tani K, Takahashi N, Takenawa T and Isobe T.
    Rb Plays a Role in Survival of Abl-dependent Human Tumor Cells as a Downstream Effector of Abl Tyrosine Kinase.
    Oncogene 25, 493-502 (2006)
  • Kajiya A, Kaji H, Isobe T, and Takeda A.
    Processing of Amyloid beta-Peptides by Neutral Cysteine Protease Bleomycin Hydrolase.
    Protein Pept Lett. 13, 119-23 (2006)
  • Kaji H and Isobe T.
    Large-scale Analysis of Glycoproteins by LC-MS Method.
    Trends Glycosci. Glycotech. 18, 313-322 (2006)
  • Nagaki K, Yamamura H, Shimada Saito, T Hisanaga S, Taoka M, Isobe T and Ichimura T.
    14-3-3 mediates phosphorylation-dependent inhibition of the interaction between the ubiquitin E3 ligase Nedd4-2 and epithelial Na+ channel.
    Biochemistry 45, 6733-6740 (2006)
  • Egawa N, Koshikawa N, Tomari T, Nabeshima K, Isobe T and Seiki M.
    Membrane type 1 matrix metalloproteinase (MT1-MMP/MMP-14) cleaves and release a 22-kDa extracellular matrix metalloproteinase inducer (EMMPRIN) fragment from tumor cells.
    J. Biol. Chem. 281, 37576-37585 (2006)
  • Hosaka M, Watanabe T, Yamauchi Y, Sakai Y, Suda M, Mizutani S, Takeuchi, T, Isobe T and Izumi T.
    A subset of p23 localized on secretary granules in pancreatic beta-cells.
    J. Histochem. Cytochem. 13, 568-597 (2006)
  • Stavreva, D A, Kawasaki M, Dundr M, Kobema K, Muller W G, Tsujimura-Takahashi T, Komatsu W, Hayano T,
    Isobe T, Raska I, Misteli T, Takahashi N and Mcnally J G.
    Potential roles for ubiqutin and the proteasome during ribosome biogenesis.
    Mol. Cell Biol. 26, 5131-5145 (2006)
  • Kaji H, Yamauchi Y, Takahashi N and Isobe T.
    Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site-specific stable isotope tagging.
    Nature Protocols 1 (6), 3019-3027 (2006)
PAGETOP

2005

  • Shinkawa T, Taoka M, Yamauchi Y, Ichimura T, Kaji H, Takahashi N and Isobe T. STEM: a software tool for large-scale proteomic data analyses.
    J Proteome Res. 4(5), 1826-31 (2005)
  • Nunomura K, Nagano K, Itagaki C, Taoka M, Okamura N, Yamauchi Y, Sugano S, Takahashi N, Izumi T and Isobe T.
    Cell Surface Labeling and Mass Spectrometry Reveal Diversity of Cell Surface Markers and Signaling Molecules Expressed in Undifferentiated Mouse Embryonic Stem Cells.
    Mol Cell Proteomics, 4(12), 1968-1976 (2005)
  • Taoka M.
    Development of large-scale protein identification technology using high-resolution LC-MS.
    Chromatography 26(2), 45-52 (2005)
    Supplementary table
  • Tokumitsu H, Hatano N, Inuzuka H, Sueyoshi Y, Yokokura S, Ichimura T, Nozaki N and Kobayashi R.
    Phosphorylation of Numb family proteins. Possible involvement of Ca2+/calmodulin-dependent protein kinases.
    J Biol Chem., 280(42), 35108-18 (2005)
  • Otsuka M, Kato N, Ichimura T, Abe S, Tanaka Y, Taniguchi H, Hoshida Y, Moriyama M, Wang Y, Shao RX, Narayan D, Muroyama R, Kanai F, Kawabe T, Isobe T and Omata M.
    Vitamin K2 binds 17beta-hydroxysteroid dehydrogenase 4 and modulates estrogen metabolism.
    Life Sci., 76(21), 2473-82 (2005)
  • Tanaka Y, Kanai F, Ichimura T, Tateishi K, Asaoka Y, Guleng B, Jazag A, Ohta M, Imamura J, Ikenoue T, Ijichi H, Kawabe T, Isobe T and Omata M.
    The hepatitis B virus X protein enhances AP-1 activation through interaction with Jab1.
    Oncogene 24, 662-71 (2005)
  • Huang M, Pring M, Yang C, Taoka M, and Zigmond SH.
    Presence of a novel inhibitor of capping protein in neutrophil extract.
    Cell Motil Cytoskeleton, 62(4), 232-43 (2005)
  • Nagano K, Itagaki C, Izumi T, Nunomura K, Soda Y, Tani K, Takahashi N, Takenawa T, and Isobe T.
    Rb plays a role in survival of Abl-dependent human tumor cells as a downstream effector of Abl tyrosine kinase.
    Oncogene 25, 492-502 (2005)
  • Higashino F, Aoyagi M, Takahashi A, Ishino M, Taoka M, Isobe T, Kobayashi M, Totsuka Y, Kohgo T, and Shindoh M.
    Adenovirus E4orf6 targets pp32/LANP to control the fate of ARE-containing mRNAs by perturbing the CRM1-dependent mechanism.
    J Cell Biol. 170(1), 15-20 (2005)
  • Kitazawa M, Yamakuni T, Song SY, Kato C, Tsuchiya R, Ishida M, Suzuki N, Adachi E, Iwashita S, Ueno S, Yanagihara N, Taoka M, Isobe T and Ohizumi Y.
    Intracellular cAMP controls a physical association of V-1 with CapZ in cultured mammalian endocrine cells.
    Biochem Biophys Res. Commun 331(1), 181-6 (2005)
  • Nagano K, Taoka M, Yamauchi Y, Itagaki C, Shinkawa T, Nunomura K, Okamura N, Takahashi N, Izumi T and Isobe T.
    Large-scale identification of proteins expressed in mouse embryonic stem cells.
    Proteomics 5(5), 1346-61 (2005)
  • Ichimura T, Yamamura H, Sasamoto K, Tominaga Y, Taoka M, Kakiuchi K, Shinkawa T, Takahashi N, Shimada S, Isobe T.
    14-3-3 proteins modulate the expression of epithelial Na+ channels by phosphorylation-dependent interaction with Nedd4-2 ubiquitin ligase.
    J Biol Chem. 280(13), 13187-94 (2005）
PAGETOP

2004

  • Oyama M, Itagaki C, Hata H, Suzuki Y, Izumi T, Natsume T, Isobe T, and Sugano S.
    Analysis of small human proteins reveals the translation of upstream open reading frames of mRNAs.
    Genome Res. 14, 2048-2052 (2004)
  • Fujimoto S, Yoshida N, Fukui T, Amanai M, Isobe T, Itagaki C, Izumi T and & Perry A C F.
    Mammalian phospholipase Czeta induces oocyte activation from the sperm perinuclear matrix.
    Dev. Biol. 274, 370-383 (2004)
  • Okamoto T, Higuchi K, Shinkawa T, Isobe T, Lorz H, Koshiba K and Kranz E.
    Identification of major proteins in maize egg cells.
    Plant Cell Physiol. 45, 1406-1412 (2004)
  • Taoka M, Yamauchi Y, Shinkawa T, Kaji H, Motohashi W, Nakayama H, Takahashi N and Isobe T.
    Only a Small Subset of the Horizontally Transferred Chromosomal Genes in Escherichia coli Are Translated into Proteins.
    Mol Cell Proteomics 3(8), 780-787(2004)
  • Ichimura T, Kubota H, Goma T, Mizushima N, Ohsumi Y, Iwago M, Kakiuchi K, Shekhar HU, Shinkawa T, Taoka M, Ito T, and Isobe T.
    Transcriptomic and proteomic analysis of a 14-3-3 gene-deficient yeast.
    Biochemistry 43(20), 6149-58 (2004)
  • Feng L, Yunoue S, Tokuo H, Ozawa T, Zhang D, Patrakitkomjorn S, Ichimura T, Saya H and Araki N.
    PKA phosphorylation and 14-3-3 interaction regulate the function of neurofibromatosis type I tumor suppressor, neurofibromin.
    FEBS Lett. 557, 275-282 （2004）
  • Yoshimura Y, Yamauchi Y, Shinkawa T, Taoka M, Donai H, Takahashi N, Isobe T and Yamauchi T.
    Molecular constituents of the postsynaptic density fraction revealed by proteomic analysis using multidimensional liquid chromatography-tandem mass spectrometry.
    J Neurochem. 88(3), 759-68 (2004)
  • Torizawa T, Shimizu M, Taoka M, Miyano H, and Kainosho M.
    Efficient production of isotopically labeled proteins by cell-free synthesis: a practical protocol.
    J Biomol NMR. 30(3), 311-25 (2004)
  • Yanagida M, Hayano T, Yamauchi Y, Shinkawa T, Natsume T, Isobe T and Takahashi N.
    Human fibrillarin forms a sub-complex with splicing factor 2-associated p32, protein arginine methyltransferases, and tubulins alpha 3 and beta 1 that is independent of its association with preribosomal ribonucleoprotein complexes.
    J Biol Chem. 279(3), 1607-14 (2004)
PAGETOP

2003

  • Taoka M, Ichimura T, Wakamiya-Tsuruta A, Kubota Y, Araki T, Obinata T and Isobe T.
    V-1, a protein expressed transiently during murine cerebellar development, regulates actin polymerization via interaction with capping protein.
    J Biol Chem. 278(8), 5864-70 (2003)
  • Kaji H, Saito H, Yamauchi Y, Shinkawa T, Taoka M, Hirabayashi J, Kasai K, Takahashi N and Isobe T.
    Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins.
    Nat Biotechnol. 21(6), 667-72 (2003)
  • Mawuenyega KG, Kaji H, Yamuchi Y, Shinkawa T, Saito H, Taoka M, Takahashi N and Isobe T.
    Large-scale identification of Caenorhabditis elegans proteins by multidimensional liquid chromatography-tandem mass spectrometry・
    J Proteome Res. 2(1), 23-35 (2003)
  • Hayano T, Yanagida M, Yamauchi Y, Shinkawa T, Isobe T and Takahashi N.
    Proteomic analysis of human Nop56p-associated pre-ribosomal ribonucleoprotein complexes. Possible link between Nop56p and the nucleolar protein treacle responsible for Treacher Collins syndrome.
    J Biol Chem. 278(36), 34309-19 (2003)
PAGETOP

2002

  • Ichimura T, Wakamiya-Tsuruta A, Itagaki C, Taoka M, Hayano T, Natsume T and Isobe T.
    Phosphorylation-dependent interaction of kinesin light chain 2 and the 14-3-3 protein.
    Biochemistry 41(17), 5566-72 (2002)
  • Hirabayashi J, Hayama K, Kaji H, Isobe T and Kasai K.Affinity capturing and gene assignment of soluble glycoproteins produced by the nematode Caenorhabditis elegans.
    J Biochem 132(1), 103-14 (2002)
  • Natsume T, Yamauchi Y, Nakayama H, Shinkawa T, Yanagida M, Takahashi N and Isobe T.
    A direct nanoflow liquid chromatography-tandem mass spectrometry system for interaction proteomics.
    Anal Chem. 74(18), 4725-33 (2002)
  • Fujiyama S, Yanagida M, Hayano T, Miura Y, Isobe T, Fujimori F, Uchida T and Takahashi N.
    Isolation and proteomic characterization of human Parvulin-associating preribosomal ribonucleoprotein complexes.
    J Biol Chem. 277(26), 23773-80 (2002)
    Erratum in: J Biol Chem. 277(44), 42418 (2002)
  • Natsume T, Taoka M, Manki H, Kume S, Isobe T and Mikoshiba K.
    Rapid analysis of protein interactions: On-chip micropurification of recombinant protein expressed in Esherichia coli.
    Proteomics 2(9), 1247-53
  • Sasaki T, Taoka M, Ishiguro K, Uchida A, Saito T, Isobe T and Hisanaga S.
    In vivo and in vitro phosphorylation at Ser-493 in the glutamate (E)-segment of neurofilament-H subunit by glycogen synthase kinase 3beta.
    J Biol Chem. 277(39), 36032-9 (2002)
  • Horiuchi T, Taoka M, Isobe T, Komano T and Inouye S.
    Role of fruA and csgA genes in gene expression during development of Myxococcus xanthus. Analysis by two-dimensional gel electrophoresis.
    J Biol Chem. 277(30), 26753-60 (2002)
  • Yoshimura Y, Shinkawa T, Taoka M, Kobayashi K, Isobe T and Yamauchi T.
    Identification of protein substrates of Ca(2+)/calmodulin-dependent protein kinase II in the postsynaptic density by protein sequencing and mass spectrometry.
    Biochem Biophys Res Commun 290(3), 948-54 (2002)
PAGETOP

2001

  • Yanagida M, Shimamoto A, Nishikawa K, Furuichi Y, Isobe T and Takahashi N.
    Isolation and proteomic characterization of the major proteins of the nucleolin-binding ribonucleoprotein complexes.
    Proteomics 1(11), 1390-404 (2001)
PAGETOP

2000

  • Kaji H, Tsuji T, Mawuenyega KG, Wakamiya A, Taoka M and Isobe T.
    Profiling of Caenorhabditis elegans proteins using two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight-mass spectrometry.
    Electrophoresis 21(9), 1755-65 (2000)
  • Taoka M, Wakamiya A, Nakayama H and Isobe T.
    Protein profiling of rat cerebella during development.
    Electrophoresis 21(9), 1872-9 (2000)
  • Yanagida M, Miura Y, Yagasaki K, Taoka M, Isobe T and Takahashi N.
    Matrix assisted laser desorption/ionization-time of flight-mass spectrometry analysis of proteins detected by anti-phosphotyrosine antibody on two-dimensional-gels of fibrolast cell lysates after tumor necrosis factor-alpha stimulation.
    Electrophoresis 21(9), 1890-8 (2000)
  • Natsume T, Nakayama H, Jansson O, Isobe T, Takio K and Mikoshiba K.
    Combination of biomolecular interaction analysis and mass spectrometric amino acid sequencing.
    Anal Chem. 7 2(17), 4193-8 (2000)
PAGETOP

著書/総説/解説

英文

PAGETOP

和文

  • 泉川桂一、石川英明、吉川治孝、礒辺俊明、高橋信弘
    “質量分析法を利用したRNAータンパク質複合体のトータル解析”
    化学と生物（印刷中）
  • 中山　洋、田岡万悟、近藤　格、礒辺俊明
    “PROTOMAP法を用いたプロテオーム解析”
    医学のあゆみ　251： 1005-1009 (2014) 
  • 石川英明、中山　洋、田岡万悟、高橋信弘、礒辺俊明
    “質量分析法を利用したＲＮＡ代謝異常症のリボヌクレオプロテオミクス解析”
    実験医学　32： 20-27 （2014）
  • 高橋信弘、礒辺俊明
    “RNAワールドとタンパク質ワールドの遭遇”（特集：RNAの時空間的動態をみる：リボヌクレオプロテオミクスによる細胞機能の解明からRNA医薬まで／企画　礒辺俊明，高橋信弘
    実験医学　28： 2564-2570 （2010）
  • 中山　洋・田岡万悟・礒辺俊明
    “質量分析法によるRNAの直接解析”（特集：RNAの時空間的動態をみる：リボヌクレオプロテオミクスによる細胞機能の解明からRNA医薬まで／企画　礒辺俊明，高橋信弘）、実験医学　28： 2581-2587 （2010）
  • 長野光司、田岡万悟
    “「質量分析法によるRNA医薬品の代謝解析」”（特集：RNAの時空間的動態をみる、リボヌクレオプロテオミクスによる細胞機能の解明からRNA医薬まで／企画　礒辺俊明，高橋信弘）
    実験医学　28, 2588-2593 (2010)
  • 田岡万悟、「技術解説：質量分析」　（特集：RNAの時空間的動態をみる、リボヌクレオプロテオミクスによる細胞機能の解明からRNA医薬まで／企画　礒辺俊明，高橋信弘）、実験医学　28：　2572 （2010）
  • 田岡万悟、中山洋、高橋信弘、礒辺俊明
    “リボヌクレオプロテオミクス研究に向けたLC-MSシステムの開発”
    細胞工学 12, 91-101 (2009)
  • 礒辺俊明・中山敬一・伊藤隆司（編）
    分子間相互作用解析ハンドブック（286 ページ） 「序論」（羊土社）（2007）
  • 田岡万悟・梶　裕之・礒辺俊明
    「オルガネラプロテオミクスの現状と細胞膜表面のプロテオーム解析」　 細胞工学（秀潤社）　25,　630-635　（2006)
  • 垣内一恵・田岡万悟・市村　徹・礒辺俊明
    「タンデムアフィニティータグ発現精製法（TAP法）」 分子間相互作用解析ハンドブック（羊土社）、pp.58-64 （2007）
  • 田岡万悟・梶　裕之・礒辺俊明
    「微量クロマトグラフィー」 ゲノム・プロテオミクスの新展開（NTS）、 pp459-467（2004）
  • 礒辺俊明・高橋信弘　
    編実験医学別冊プロテオーム解析マニュアル（羊土社 ） (2004)
  • 新川高志・梶裕之・礒辺俊明
    「試料の前処理法」実験医学別冊プロテオーム解 析マニュアル（羊土社）、pp62-69（2004）
  • 山内芳雄・田岡万悟・夏目徹・早野俊哉・高橋信弘・礒辺俊明
    「タンパク質複合体解析のためのダイレクトナノフロー LC-MS法」 実験医学別冊プロテオーム解析マニュアル（羊土社）、 pp81-91（2004）
  • 田岡万悟・山内芳雄・梶裕之・礒辺俊明
    「タンパク質の大規模解析のための二次元LC-MS法」 実験医学別冊プロテオーム解析マニュアル（羊土社）、 pp92-100（2004）
  • 新川高志・田岡万悟・梶裕之・礒辺俊明
    「大規模プロテオーム解析のためのデータ処理法」 実験医学別冊プロテオーム解析マニュアル（羊土社）、 pp101-110（2004）
  • 梶裕之・礒辺俊明
    「糖鎖修飾タンパク質の大規模同定法」 実験医学別冊プロテオーム解析マニュアル（羊土社）、 pp150-157（2004）
  • 市村徹・垣内一恵・笹本要・礒辺俊明
    「エピトープタグ発現法」 実験医学別冊プロテオーム解析マニュアル（羊土社）、 pp168-179（2004）
  • 梶　裕之
    「グライコプロテオーム解析(ミニレビュー)」 生化学 76,　363-368、日本生化学会 (2004)
  • 田岡万悟、梶　裕之、山内芳雄、礒辺俊明
    「プロテオーム研究の新戦略と創薬」 月間薬事、46巻7号、pp1251-1256　(2004)
  • 礒辺俊明、田岡万悟、山内芳雄、新川高志、梶　裕之、高橋信弘
    「液体クロマトグラフィーを基礎にしたプロテオーム解析システム」 PNE増刊号『バイオ高性能機器・新技術利用マニュアル』共立出版、pp1871-1878（2004）
  • 成松　久 、亀山昭彦 、梶　裕之
    「グライコミクス解析システム」 PNE増刊号『バイオ高性能機器・新技術利用マニュアル』 共立出版、pp1900-1906（2004）
  • 新川高志・礒辺俊明
    「プロテオミクス研究のためのLC-MS/MS法」 メディカルサイエンスダイジェスト　29、 pp136-137（2003）
  • 礒辺俊明
    「プロテオミクスの戦略と動向」 科学（岩波書店）、pp443-444（2003）
  • 梶　裕之・山内芳雄・礒辺俊明
    「IGOT法を利用したN型糖タンパク質の大規模解析」 Bioベンチャー11 ・12（羊土社）、pp58-59（2003）
  • 市村徹
    「シグナル伝達経路のプロテオミクス解析」 臨床神経科学（中外医学社）、pp1226-1227（2003）
PAGETOP

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PAGETOP