研究履歴
1994年 筑波大学博士課程体育科学研究科修了
1994年 筑波大学応用生物化学系遺伝子実験センター
国際科学振興財団 専任研究員
1996年 筑波大学応用生物化学系遺伝子実験センター
日本学術振興会 特別研究員 –PD
1999年 ジョスリン糖尿病センター(米国), ポストドクトラルフェロー
ハーバード大学医学部(米国), ポストドクトラルフェロー
日本学術振興会 海外特別研究員
2001年 ジョスリン糖尿病センター, Research Associate
ハーバード大学医学部, Instructor of Medicine
2008年 首都大学東京 大学院人間健康科学研究科
ヘルスプロモーションサイエンス学域 教授
1999年- Red Sox Nation (Boston), Citizen
専門分野
これまで、運動生理学、分子生物学、代謝内分泌学の研究室で、それぞれの研究に従事してきました。現在は、運動の大事な要素である「筋収縮」に焦点を当て、骨格筋と筋収縮の新生物学を展開しています。運動によって健康がもたらされる仕組みを、細胞および細胞内分子のレベルで解明することを目標にしています。
研究者を志望する皆さんへ
研究が大好きな人、研究に強い憧れがある人、研究者になりたいと思っている人。そんな人たちの受け皿となれればと思っています。藤井・眞鍋研のメンバーは皆、自分らしい新発見をして、それを世界に向け発信することに真剣です。卒業研究、大学院受験、ポスドク研究を考えている人は、わたし達の研究活動をぜひ見に来てください。相談や見学だけでなく、ゼミ体験参加も随時行っています。（042-677-2966,
fujiin@tmu.ac.jp）
注意
異なる視点からのプロフィールはこちら
研究履歴に対する論文情報
Miyatake S, Manabe Y, Inagaki A, Furuichi Y, Takagi M, Taoka M, Isobe T, Hirota K, Fujii NL. Macrophage migration inhibitory factor diminishes muscle glucose transport induced by insulin and AICAR in a muscle type-dependent manner. Biochem.Biophys.Res.Commun., 444, 496-501, 2014
Goto-Inoue N, Yamada K, Inagaki A, Furuichi Y, Ogino S, Manabe Y, Setou M, Fujii NL. Lipidomics analysis revealed the phospholipid compositional changes in muscle by chronic exercise and high-fat diet. Sci.Rep., 3, 3267, 2013
Tanaka A, Woltjen K, Miyake K, Hotta A, Ikeya M, Yamamoto T, Nishino T, Shoji E, Sehara-Fujisawa A, Manabe Y, Fujii N, Hanaoka K, Era T, Yamashita S, Isobe K, Kimura E, Sakurai H. Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro. PLoS One,8, e61540, 2013
Tsuchiya M*, Manabe Y* (*contributed equally), Yamada K, Furuichi Y, Hosaka
M, Fujii NL. Chronic exercise enhances insulin secretion ability
of pancreatic islets without change in insulin content in non-diabetic
rats. Biochem.Biophys.Res.Commun., 430(2):676-682, 2013
Manabe Y, Gollisch KSC, Holton L, Kim Y, Brandauer J, Fujii NL, Hirshman MF, Goodyear LJ.
Exercise training-induced adaptations associated with increases in skeletal muscle glycogen content. FEBS J., 280(3):916-926, 2013
Manabe Y, Miyatake S, Takagi M, Nakamura M, Okeda A, Nakano T, Hirshman
MF, Goodyear LJ, Fujii NL. Characterization of an Acute Muscle Contraction
Model using Cultured C2C12 Myotubes. PLoS ONE,7(12):e52592, 2012
Goto-Inoue N, Manabe Y, Miyatake S, Ogino S, Morishita A, Hayasaka T, Masaki N, Setou M, Fujii NL.
Visualization of dynamic change in contraction-induced
lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization
imaging mass spectrometry. Anal.Bioanal.Chem., 403(7):1863-1867,2012
Henstridge DC, Bruce CR, Pang CP, Lancaster GI, Allen TL, Estevez E, Gardner
T, Weir JM, Meikle PJ, Lam KS, Xu A, Fujii N, Goodyear LJ, Febbraio MA.
Skeletal muscle-specific overproduction of constitutively activated c-Jun
N-terminal kinase (JNK) induces insulin resistance in mice. Diabetologia.
55(10):2769-2778. 2012
Toyoda T, An D, Witczak CA, Koh HJ, Hirshman MF, Fujii N, Goodyear LJ. Myo1c
regulates glucose uptake in mouse skeletal muscle. J.Biol.Chem.
2011, 286(6):4133-40.
Sugita S, Kamei Y, Akaike F, Suganami T, Kanai S, Hattori M, Manabe Y,
Fujii N, Takai-Igarashi T, Tadaishi M, Oka J, Aburatani H, Yamada T, Katagiri
H, Kakehi S, Tamura Y, Kubo H, Nishida K, Miura S, Ezaki O, Ogawa Y. Increased
systemic glucose tolerance with increased muscle glucose uptake in transgenic
mice overexpressing RXRγ in skeletal muscle. PLoS One. 2011;6(5):e20467
Koh HJ, Toyoda T, Fujii N, Jung MM, Rathod A, Middelbeek RJ, Lessard SJ,
Treebak JT, Tsuchihara K, Esumi H, Richter EA, Wojtaszewski JF, Hirshman
MF, and Goodyear LJ, Sucrose nonfermenting AMPK-related kinase (SNARK)
mediates contraction-stimulated glucose transport in mouse skeletal muscle.
Proc Natl.Acad.Sci. USA. 2010; 107(35): 15541-6.
Jessen N, Koh HJ, Folmes CD, Wagg C, Fujii N, Løfgren B, Wolf CM, Berul
CI, Hirshman MF, Lopaschuk GD, and Goodyear LJ, Ablation of LKB1
in the heart leads to energy deprivation and impaired cardiac function.
Biochim Biophys Acta. 2010; 1802(7-8): 593-600.
An D, Toyoda T, Taylor EB, Yu H, Fujii N, Hirshman MF, and Goodyear LJ,
TBC1D1 regulates insulin- and contraction-induced glucose transport in
mouse skeletal muscle. Diabetes. 2010; 59(6): 1358-65.
Witczak CA, Jessen N, Warro DM, Toyoda T, Fujii N, Anderson ME, Hirshman
MF, and Goodyear LJ, CaMKII regulates contraction- but not insulin-induced
glucose uptake in mouse skeletal muscle. Am.J.Physiol. Endocrinol.Metab.
2010; 298(6): E1150-60.
Fujii N. Molecular mechanism of glucose transport in skeletal muscle.
Adv.Exer.Sport.Physiol. 2008; 14(3): 45-47
Fujii N, Ho RC, Manabe Y, Jessen N, Toyoda T, Holland WL, Summers SA, Hirshman
MF, Goodyear LJ. Ablation of AMP-activated protein kinase alpha2
activity exacerbates insulin resistance induced by high-fat feeding of
mice. Diabetes. 2008 Nov;57(11):2958-66
Kramer HF, Taylor EB, Witczak CA, Fujii N, Hirshman MF, Goodyear LJ,
Calmodulin-binding domain of AS160 regulates contraction- but not insulin-stimulated
glucose uptake in skeletal muscle. Diabetes 2007; 56(12):2854-62.
Ho RC, *Fujii N, Witters LA, Hirshman MF, Goodyear LJ. Dissociation
of AMP-activated protein kinase and p38 mitogen-activated protein kinase
signaling in skeletal muscle
Rockl KS, Hirshman MF, Brandauer J, Fujii N, Witters LA, Goodyear LJ.
Skeletal muscle adaptation to exercise training: AMP-activated protein
kinase mediates muscle fiber type shift. Diabetes 2007; 56(8):2062-9.
Fujii N, Seifert MM, Kane EM, Peter LE, Ho RC, Winstead S, Hirshman MF,
Goodyear LJ. Role of AMP-activated protein kinase in exercise capacity,
whole body glucose homeostasis, and glucose transport in skeletal suscle.
- Insight from analysis of a transgenic mouse model - Diabetes Res.Clin.Pract.
2007; 77:S92-8.
Fujii N, Jessen N, Goodyear LJ. AMP-activated Protein Kinase and
the Regulation of Glucose Transport. Am.J.Physiol. (Endcrinol.Metab.)
2006; 291(5):E867-77
Witczak CA, Hirshman MF, Jessen N, Fujii N, Seifert MM, Brandauer J, Hotamisligil
GS, Goodyear LJ. JNK1 deficiency does not enhance muscle glucose
metabolism in lean mice. Biochem.Biophys.Res.Commun. 2006; 350(4):1063-8
Koh H, Arnolds DE, Fujii N, Hirshman MF, Goodyear LJ. Skeletal muscle-selective
knockout of LKB1 increases insulin sensitivity, improves glucose homeostasis,
and decreases TRB3. Mol.Cell Biol. 2006; 26(22):8217-27
Kramer HF, Witczak CA, Taylor EB, Fujii N, Hirshman MF, Goodyear LJ.
AS160 regulates insulin- and contraction-stimulated glucose uptake in mouse
skeletal muscle. J.Biol.Chem. 2006; 281(42):31478-85
Kramer HF, Witczak CA, Fujii N, Jessen N, Taylor EB, Arnolds DE, Sakamoto
K, Hirshman MF, Goodyear LJ. Distinct signals regulate AS160 phosphorylation
in response to insulin, AICAR, and contraction in mouse skeletal muscle.
Diabetes 2006; 55(7):2067-76
Sakamoto K, Arnolds DE, Fujii N, Kramer HF, Hirshman MF, Goodyear LJ.
Role of Akt2 in contraction-stimulated cell signaling and glucose uptake
in skeletal muscle. Am.J.Physiol. (Endocrinol.Metab.) 2006; 291(5):E1031-7
Yu H, Hirshman MF, Fujii N, Pomerleau JM, Peter LE, Goodyear LJ.
Muscle-specific overexpression of wild type and R225Q mutant AMP-activated
protein kinase gamma3 Subunit differentially regulates glycogen accumulation.
Am.J.Physiol. (Endocrinol.Metab.) 2006; 291(3):E557-65
Aschenbach WG, Ho RC, Sakamoto K, Fujii N, Li Y, Kim YB, Hirshman MF, Goodyear
LJ. Regulation of dishevelled and beta-catenin in rat skeletal muscle:
an alternative exercise-induced GSK-3beta signaling pathway. Am.J.Physiol.
(Endocrinol.Metab.) 2006; 291 (1): E152-8.
Fujii N, Hirshman MF, Kane EM, Ho RC, Peter LE, Seifert MM, Goodyear LJ.
AMP-activated protein kinase alpha2 activity is not essential for contraction-and
hyperosmolarity-induced glucose transport in skeletal muscle. J.Biol.Chem.
2005; 280 (47): 29033-29041.
Pold R, Jensel LS, Jessen N, Buhl ES, Schmitz O, Flyvbjerg A, Fujii N,
Goodyear LJ, Gotfredsen CF, Brand CL, Lund S. Long-term AICAR administration
and exercise prevents diabetes in ZDF rats. Diabetes 2005; 54(4):928-34<
Musi N, Hirshman MF, Arad M, Xing Y, Fujii N, Pomerleau J, Ahmad F, Berul
CI, Seidman JG, Tian R, Goodyear LJ. Functional Role of AMP-activated
protein kinase in the heart during exercise. FEBS Lett. 2005; 579(10):2045-50
Fujii N, Aschenbach WG, Musi N, Hirshman MF, Goodyear LJ. Regulation
of glucose transport by the AMP-activated protein kinase. Proc.Nutr.Soc.
2004; 63: 205-210
Alcazar O, Ho R, Fujii N, Goodyear LJ. cDNA cloning and functional
characterization of a novel splice variant of c-Cbl associated protein
(CAP) from mouse skeletal muscle. Biochem.Biophys.Res.Comm. 2004;
317(1): 285-93
Fujii N, Boppart MD, Dufresne SD, Crowley PF, Jozsi AC, Sakamoto K, Kim
S, Miyazaki H, Hirshman MF, Goodyear LJ. Overexpression or ablatiation
of JNK in skeletal muscle has no effect on glycogen synthase activity.
Am.J.Physiol. (Cell Physiol.) 2004; 287(1): C200-8
Ho R, Alcazar O, Fujii N, Hirshman MF, Goodyear LJ. p38gamma MAPK
regulation of glucose transporter expression and glucose uptake in L6 myotubes
and mouse skeletal muscle. Am.J.Physiol. (Regul.Integr.Comp.Physiol.)
2004; 286(2): R342-9
Yu H, Fujii N, Hirshman MF, Pomerleau JM, Goodyear LJ. Cloning and
characterization of mouse 5’AMP-activated protein kinase gamma3 subunit.
Am.J.Physiol. (Cell Physiol.) 2004; 286(2): C283-92
Iemitsu M, Miyauchi T, Maeda S, Sakai S, Fujii N, Miyazaki H, Kakinuma
Y, Matsuda M, Yamaguchi, I. Cardiac hypertrophy by hypertension and
exercise training exhibits different gene expression of enzymes in energy
metabolism.
Hypertens.Res.2003; 26(10): 829-37
Xing Y, Musi N, Fujii N, Zou L, Luptak I, Hirshman MF, Goodyear LJ, Tian
R. Glucose metabolism and energy homeostasis in mouse hearts overexpressing
dominant negative alpha 2 subunit of AMP-activated protein kinase.
J.Biol.Chem.2003; 278(31): 28372-28377
Kaneto H, Xu G, Fujii N, Kim S, Bonner-Weir S, Weir GC. Involvement
of c-Jun N-terminal kinase in oxidative stress-mediated suppression of
insulin gene expression. J.Biol.Chem. 2002; 277(33): 30010-30018
Aschenbach WG, Hirshman MF, Fujii N, Sakamoto K, Howlett KF, Goodyear LJ.
Effect of AICAR treatment on glycogen metabolism in skeletal muscle.
Diabetes 2002; 51(3): 567-573
Iemitsu M, Miyauchi T, Maeda S, Sakai S, Kobayashi T, Fujii N, Miyazaki
H, Matsuda M, Yamaguchi I. Physiological and pathological cardiac
hypertrophy induce different molecular phenotypes in the rat. Am.J.Physiol.
(Regul Integr Comp Physiol ) 2001; 281(6): R2029-2036
Zhou G, Myers R, Li Y, Chen Y, Shen X, Fenyk-Melody J, Wu M, Ventre J,
Doebber T, Fujii N, Musi N, Hirshman MF, Goodyear LJ, Moller DE.
Role of AMP-activated protein kinase in mechanism of metformin action.
J.Clin.Invest. 2001; 108(8): 1167-1174
Musi N, Fujii N, Hirshman MF, Ekberg I, Froberg S, Ljungqvist O, Thorell
A, Goodyear LJ. AMP-activated protein kinase (AMPK) is activated
in muscle of subjects with type 2 diabetes during exercise. Diabetes
2001; 50(5): 921-927
Musi N, Hayashi T, Fujii N, Hirshman MF, Witter LA, Goodyear LJ.
AMP-activated protein kinase activity and glucose uptake in rat skeletal
muscle. Am.J.Physiol. (Endocrinol. Metab.) 2001; 280 (5): E677-68
Higaki Y, Hirshman MF, Fujii N, Goodyear LJ. Nitric oxide increases
glucose uptake through a mechanism that is distinct from the insulin and
contraction pathways in rat skeletal muscle. Diabetes 2001; 50(2):
241-247
Fujii N, Hayashi T, Hirshman MF, Smith JT, Habinowski SA, Kaijser L, Mu
J, Ljungqvist O, Birnbaum MJ, Witters LA, Thorell A, Goodyear LJ.
Exercise induces isoform-specific increases in 5’ AMP-activated protein
kinase in human skeletal muscle. Biochem.Biophys.Res.Comm. 2000; 273(3):
1150-1155
Hayashi T, Hirshman M, Fujii N, Habinowski SA, Witters LA, Goodyear LJ.
Metabolic stress and altered glucose transport: activation of AMP-activated
protein kinase as a unifying coupling mechanism. Diabetes 2000; 49(4):
527-531
Tanaka M, Tsuchida S, Imai T, Fujii N, Miyasaki H, Ichiki T, Naruse M,
Inagami T. Vascular response to angiotensin II is exaggerated through
an upregulation of AT1 receptor in AT2 knockout mice. Biochem.Biophys.Res.Commun.
1999; 258(1): 194-19
Kotani E, Sugimoto M, Kamata H, Fujii N, Saitoh S, Usuki S, Kubo T, Song
K, Miyazaki M, Murakami K, Miyazaki H. Biological roles of angiotensin
II via its type2 receptor during rat follicle atresia. Am.J.Physiol.
(Endocrinol. Metab.) 1999; 276(39): E25-33
Fujii N, Nagashima S, Yukawa K, Miyauchi T, Maki S, Sakai S, Murakami K,
Miyazaki H. Hypotensive effects of an angiotensin II type 1 receptor
antagonist differ between exercised and sedentary rats aged from 4 to 19
weeks. Jpn.J.Physiol. 1998(3); 48: 215-218
Fujii N, Homma S, Yamazaki F, Sone R, Ikegami H, Murakami K, Miyazaki H.
b-adrenergic receptor number in human lymphocytes is inversely correlated
with aerobic capacity. Am.J.Physiol. (Endcrinol. Metab.) 1998; 274(37):
E1106-1112
Fujii N, Shibata T, Homma S, Ikegami H, Murakami K, Miyazaki H. Exercise-induced
changes in b-adrenergic receptor mRNA level measured by competitive RT-PCR.
J.Appl.Physiol. 1997; 82(6): 1926-1931
Fukuoka Y, Shigematsu M, Itoh M, Fujii N, Homma S, Ikegami H. Effects
of football training on ventilatory and gas exchange kinetics to sinusoidal
work load. J.Sport.Med.Phys.Fit. 1997; 37(3): 161-167
Fujii N, Shibata T, Yamazaki F, Sone R, Fukuoka Y, Nabekura Y, Ikegami
H, Murakami K, Miyazaki H. Exercise-induced change in b-adrenergic
receptor number in lymphocytes from trained and untrained men. Jpn.J.Physiol.
1996; 46(5): 389-395
Sone R, Yamazaki F, Fujii N, Fukuoka Y, Ikegami H. Respiratory
variability in R-R interval during sinusoidal exercise. Eur.J.Appl.Physiol.
1996; 75(1): 39-46
Yamazaki F, Fujii N, Sone R, Ikegami H. Responses of sweating and
body temperature to sinusoidal exercise in physically trained men.
J.Appl.Physiol. 1996; 80(2): 491-495
Sone R, Yamazaki F, Homma S, Fujii N, Ikegami H. Effects of sensory
stimulation on respiratory cardiac cycle variability in humans.
Eur.J.Appl.Physiol. 1995; 70(5): 394-400
Fujii N, Tanaka M, Ohnishi J, Yukawa K, Takimoto E, Shimada S, Naruse M,
Sugiyama F,Yagami K, Murakami K, Miyazaki H. Alterations of
angiotensin II receptor contents in hypertrophied hearts. Biochem.Biophys.Res.Comm.
1995; 212(2): 326-333
Yamazaki F, Fujii N, Sone R, Ikegami H. Mechanisms of potentiation
in sweating induced by long-term physical training. Eur.J.Appl.Physiol.
1994; 69(3): 228-232
Homma S, Fujii N, Sone R, Yamazaki F, Eda H, Ikegami H. Muscle circulation
and metabolic activity during exhaustive cycle exercise studied by near-infrared
spectroscopy. J.Exerc.Sci. 1993; 3: 49-56
Yamazaki F, Sone R, Fujii N, Ikegami H. Comparison of sweating response
during exercise and recovery in terms of sweating rate and body temperature.
Int.J.Biometeorol. 1993; 37(4): 212-217
Yamazaki F, Sone R, Fujii N, Homma S, Ikegami H. An improved method
for measurement of sweat expulsions during profuse sweating. Jpn.J.Physiol.
1993; 42(6): 991-996
Fujii N, Miyazaki H, Homma S, Ikegami H. Dynamic exercise induces
translocation of b-adrenergic receptors in human lymphocytes. Acta
Physiol.Scand. 1993; 148(4): 463-464