NLS utility page

1. NMR measurement

1-1. Pulse sequences
These pulse programs were tested in the following environment.
Spectrometer: Bruker AVANCE(I)-600
Software: TOPSPIN 1.3 or 2.1

CT: constant time
C/N: 13C/15N-labelled
D/C/N: 2H/13C/15N-labelled

1-1-1. Backbone experiments

	conventional	TOPSPIN 1.3	TOPSPIN 2.1
3D HNCA (15N-CT) for C/N proteins	i_hncar	i_hncar_nls2	i_hncar_nls4
3D HN(CO)CA (15N-CT) for C/N proteins	i_hncocar	i_hncocar_nls2	i_hncocar_nls4
3D CBCANH (13C/15N-CT) for C/N proteins	i_cbcanh	i_cbcanh_nls2	i_cbcanh_nls4
3D CBCA(CO)NH (13C/15N-CT) for C/N proteins	i_cbcaconh	i_cbcaconh_nls2	i_cbcaconh_nls4
3D HN(CA)CO (15N-CT) for C/N proteins	i_hncacor	i_hncacor_nls2	i_hncacor_nls4
3D HNCO (15N-CT) for C/N proteins	i_hncor	i_hncor_nls2	i_hncor_nls4

1-1-2. Side-chain experiments

1-1-3. NOE experiments



1-2. Variable counter (VC) lists for controling sampling points.

1-2-1. A computer program (a shell script + a C-language program) for generating VC lists and sampling lists for AZARA processing.

        3dnls_gen.tgz

1-2-2. A typical VC list for nonlinear sampling.

        64e22c_176.vc


2. AZARA processing

2-1. Scripts
These scripts were tested in the following environment.
Data: Bruker AVANCE-I.
Software: AZARA v2.7 (littleendian) / LINUX PC.

2-1-1. A typical "par" file for input data to process.

        hncoca_nls4.ser.par

2-1-2. A typical script for processing of dim 1.

        hncoca_nls4_d1.scr

2-1-3. A typical script for 2D MaxEnt processing of nonlinearly acquired data.

        hncoca_nls4_mem.scr

2-2. Sampling lists

2-1-1. A typical sampling list for MaxEnt processing of nolinearly acquired data.

        64e22c_176.lst

Last updated: 5 January 2010